Subject Area
Biological Oceanography, Marine Ecology
Activities
Marine biologist currently studying the productivity of mangrove dominated tropical estuaries and deep-sea ecology with specialization in marine microbiology and molecular analyses of bacteria. Proficient in DNA extraction, PCR, 16S rRNA sequencing, molecular taxonomy,bacterial productivity and respiration studies, stable carbon and nitrogen isotopic and extracellular enzymatic studies.
Skills
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Molecular biology: Bacterial DNA extraction and purification,
Polymerase Chain Reaction (PCR), Agarose Gel electrophoresis, Capillary sequencing (Sanger), screening of
extracellular enzymes.
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Bioinformatics and statistical
analyses: SPSS version 23, PAST 3, CLUSTAL X, MOTHUR, ANOVA, Pearson’s
correlation coefficient, principal component analysis, linear regression
analysis, multiple regression
analysis, and post-hoc Dunnett’s test.
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Microbiology: Optical, Epifluorescence microscopy, microbial culture inoculation, isolation, biochemical characterization, bacterial cultures handling and maintenance.
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Instrumentation: Coulometer (Total
Inorganic Carbon in marine sediments), Total organic carbon analyser,
CN Elemental Analyzer (Total Carbon and Total Nitrogen in marine
sediments), Autoanalyser (Nitrate, Nitrite, Phosphate, Silicate, Ammonia
in seawater), Graphite furnace and flame atomic absorption spectroscopy (Iron,
Copper, Zinc, Chromium), Direct mercury analyser, Scintillation counter
(Radioactive measurements), Isotope
ratio mass spectroscopy (Carbon (δ13 C) and nitrogen (δ15 N)
isotopes), Elemental analyser (C:N ratio).