https://dev.oceanexpert.org/expert/27223

PhD

Fisheries
Aquaculture

 Bacterial and Viral pathogenesis  Vaccine development and antiviral drugs  Drugs from sea  Phramacological Important Biosurfactants

Indian Ocean

PCR, RT-PCR, cloning, Northen Blot, Construction of cDNA and SMART c DNA Libraries, In situ Hybridization using labeled probes and Immunohisto chemistry, Small animal breading and handling Recombinant protein expression in E.coli by various induction methods, Recombinant protein purification using Nickel column for His tag and Glutathione Sepharose for GST fusion protein, Protein characterization by SDS-PAGE, Native PAGE electrophrosis. Western blot analysis polyclonal antibody and ELISA. Poly-clonal antibody development in rabbit using the purified recombinant protein expressed in E.coli.

English

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